Construction of an equalized cDNA library from Arabidopsis thaliana
نویسندگان
چکیده
منابع مشابه
A cDNA clone for an ATP-sulfurylase from Arabidopsis thaliana.
Sulfate is the predominant sulfur source for plants. After uptake from the soil via specific transport proteins into plant roots, sulfate is activated by the enzyme ATP-sulfurylase to APS. ATP-sulfurylase activity in plants is detectable in green tissues and roots (Ellis, 1969; Lunn et al., 1990). Isoforms of the enzyme are localized in plastids and in the cytosol (Lunn et al., 1990; Renosto et...
متن کاملYeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...
متن کاملساخت و تکثیر cDNA از mRNA ژن رمز گردان آنزیم P5CS گیاه Arabidopsis thaliana
گیاهان وقتی در معرض خشکی و شوری قرار می گیرند با تنش اسمزی مواجه می شوند که برای مقابله با تنش اسمزی ایجاد شده تجمع اسمولیتهایی همچون پرولین ، گلیسین بتانین و یا سایر ترکیبات مشابه را افزایش می دهند.در مسیر بیوسنتز این اسمولیتها آنزیمهایی دخالت دارند که بعضی از آنها کلیدی به حساب می‌آیند و با تغییر در تظاهر ژنهایی که این آنزیمها را کد می کنند می توان فراوردة نهایی که اسمولیت مورد نظر می‍‍باشد ...
متن کاملMolecular cloning of a cysteine synthase cDNA from Arabidopsis thaliana.
Cys biosynthesis in higher plants occurs by a process similar to that known in microorganisms (Kredich, 1987). Cys synthase [O-acetylserine (thio1)-lyase, EC 4.2.99.81 catalyzes the formation of Cys from O-acetylserine and free or carrier-bound sulfide. Cys synthase has been purified from various plants and microorganisms and has been shown to have a molecular mass of 52 to 70 kD. The plant enz...
متن کاملFunctional cloning of an Arabidopsis thaliana cDNA encoding cycloeucalenol cycloisomerase.
Plants and certain protists use cycloeucalenol cycloisomerase (EC ) to convert pentacyclic cyclopropyl sterols to conventional tetracyclic sterols. We used a novel complementation strategy to clone a cycloeucalenol cycloisomerase cDNA. Expressing an Arabidopsis thaliana cycloartenol synthase cDNA in a yeast lanosterol synthase mutant provided a sterol auxotroph that could be genetically complem...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1995
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1995.08050771.x